The very opening shot is a fade-in to a medium close-up on a man and a women embracing, both of which have red eyes to indicate that they have been crying. The first line the audience hears confirms this. As the woman says "I love you, Wade Wilson. We can fight this." the camera shot jump cuts to a close up of them holding hands. This displays their close relationship, but the opening line and slow build of dramatic music implies there is something seriously wrong.
However, Wade's reply is darkly humourous and laced with sarcasm. This gives an insight into his character. It also shows an x-ray of the problems he's joking about, giving the audience an insight into the severity.
The intense music, combined with the narrative, and short clips of Wilson's backstory creates tension and excitement- the audience want to know the outcome of all these experiments.
It's obvious that the treatment Wilson gets is painful- one scientist who helps with the procedure says "One thing that never survives this place is a sense of humour." Wilson retaliates to this with some insulting comparisons to celebrities, which earns him a punch. This shows that he might be a bit too laid back for his own good, and that it will put him in trouble if he isn't careful.
At fifty seconds the atmosphere quickly changes. The Marvel logo flashes up and rap music starts. Initially the audience believe this is a non-diegetic soundtrack to set the mood again, but when the scene opens with a close-up on a radio with a sticker saying "Wade" in red crayon it is revealed that it's something that Wilson is listening to and muttering along to as he draws. His drawing is done in crayon, like the label of his radio, and in a very cartoon-y style. It's a scribbled doodle of Deadpool shooting a bad guy in the head, who is saying "Ouchie!" This makes it obvious that Wilson has a child-like mind and sense of humour sometimes.

